PhoA

• Description: alkaline phosphatase A
  
  

Gene name	phoA
Synonyms	phoAIV
Essential	no
Product	alkaline phosphatase A
Function	aquisition of phosphate upon phosphoate starvation
MW, pI	50 kDa, 9.926
Gene length, protein length	1383 bp, 461 aa
Immediate neighbours	spoVR, lytE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A phosphate monoester + H₂O = an alcohol + phosphate (according to Swiss-Prot)

• Protein family: alkaline phosphatase family (according to Swiss-Prot)

• Paralogous protein(s): PhoB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed

Database entries

• Structure:

• Swiss prot entry: P19406

• KEGG entry: [3]

• E.C. number: 3.1.3.1

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

H Antelmann, C Scharf, M Hecker
Phosphate starvation-inducible proteins of Bacillus subtilis: proteomics and transcriptional analysis.
J Bacteriol: 2000, 182(16);4478-90
[PubMed:10913081] [WorldCat.org] [DOI] (P p)

W Liu, Y Qi, F M Hulett
Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity.
Mol Microbiol: 1998, 28(1);119-30
[PubMed:9593301] [WorldCat.org] [DOI] (P p)

F M Hulett, J Lee, L Shi, G Sun, R Chesnut, E Sharkova, M F Duggan, N Kapp
Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilis.
J Bacteriol: 1994, 176(5);1348-58
[PubMed:8113174] [WorldCat.org] [DOI] (P p)

1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed