pheT

pheT
168

phenylalanyl-tRNA synthetase (beta subunit)

locus
BSU_28630
Molecular weight
87.76 kDa
pI
4.86
Protein length
Gene length
function
translation
product
phenylalanyl-tRNA synthetase (beta subunit)
essential
yes
ec
6.1.1.20
synonyms
pheT

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0073 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,927,008 2,929,422
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
ATP + L-phenylalanine + tRNAPhe --> AMP + diphosphate + H+ + L-phenylalanyl-tRNAPhe (according to UniProt)
Protein family
phenylalanyl-tRNA synthetase beta subunit family (single member, according to UniProt)
[wiki|Domains]
[wiki|tRNA-binding domain] (aa 40-155) (accordinig to UniProt)
B5 domain (aa 409-484) (according to UniProt)
FDX-ACB domain (aa 710-803) (according to UniProt)
Structure
[PDB|1PYS] (the [protein|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[protein|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT] complex from ''Thermus thermophilus hb8 mutant'', 37% identity, 56% similarity) [Pubmed|7664121]
[AF|P17922]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[gene|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT]
description
[Pubmed|2127701]
regulation
induced by phenylalanine limitation ([wiki|T-box]) [Pubmed|19258532]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, via tRNA controlled [wiki|RNA switch], in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2127701], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]→[gene|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT]

2024-03-17 04:07:19

Jstuelk

65

604c7f0822297bd3345163e5edd4c0c2a40eca4b

FF94A47A73789CF861E1FCC8CDB2858699A5CEE2

References
19258532,2127701,15378759,36815589

B255B87EB7A850D1C6B7930A3731DAD6AE0A107D

Page visits: 4076

Time of last update: 2024-03-28 05:32:19

Author of last update: Jstuelk