Difference between revisions of "PheT"
| Line 34: | Line 34: | ||
<br/><br/><br/><br/><br/><br/> | <br/><br/><br/><br/><br/><br/> | ||
| + | |||
| + | = [[Categories]] containing this gene/protein = | ||
| + | {{SubtiWiki category|[[translation]]}}, | ||
| + | {{SubtiWiki category|[[essential genes]]}} | ||
| + | |||
| + | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[T-box]]}} | ||
=The gene= | =The gene= | ||
| Line 55: | Line 62: | ||
| − | + | ||
| − | |||
| − | |||
=The protein= | =The protein= | ||
Revision as of 22:13, 8 December 2010
- Description: phenylalanyl-tRNA synthetase (beta subunit)
| Gene name | pheT |
| Synonyms | |
| Essential | yes PubMed |
| Product | phenylalanyl-tRNA synthetase (beta subunit) |
| Function | translation |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 87 kDa, 4.864 |
| Gene length, protein length | 2412 bp, 804 aa |
| Immediate neighbours | rnhC, pheS |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28630
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
- Protein family: Type 1 subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P17922
- KEGG entry: [3]
- E.C. number: 6.1.1.20
Additional information
Expression and regulation
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701]
[WorldCat.org]
[DOI]
(P p)
