PheT

• Description: phenylalanyl-tRNA synthetase (beta subunit)
  
  

Gene name	pheT
Synonyms
Essential	yes PubMed
Product	phenylalanyl-tRNA synthetase (beta subunit)
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	87 kDa, 4.864
Gene length, protein length	2412 bp, 804 aa
Immediate neighbours	rnhC, pheS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28630

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)

• Protein family: Type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PheS-PheT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed

• UniProt: P17922

• KEGG entry: [3]

• E.C. number: 6.1.1.20

Expression and regulation

• Operon: pheS-pheT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by phenylalanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)