PheS

• Description: phenylalanyl-tRNA synthetase (alpha subunit), universally conserved protein
  
  

Gene name	pheS
Synonyms
Essential	yes PubMed
Product	phenylalanyl-tRNA synthetase (alpha subunit)
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	38 kDa, 5.083
Gene length, protein length	1032 bp, 344 aa
Immediate neighbours	pheT, ysgA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28640

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)

• Protein family: Phe-tRNA synthetase alpha chain type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PheS-PheT

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1PYS (from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed

• UniProt: P17921

• KEGG entry: [3]

• E.C. number: 6.1.1.20

Additional information

universally conserved protein

Expression and regulation

• Operon: pheS-pheT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by phenylalanine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

A A Brakhage, M Wozny, H Putzer
Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.
Biochimie: 1990, 72(10);725-34
[PubMed:2127701] [WorldCat.org] [DOI] (P p)