pheA

168

prephenate dehydratase

locus: BSU_27900

Molecular weight: 31.75 kDa

pI: 5.89

Protein length: 285 aa

Gene length: 858 bp

function: biosynthesis of phenylalanine

product: prephenate dehydratase

essential: no

ec: 4.2.1.51

synonyms: pheA

List of homologs in different organisms, belongs to COG0077 (Galperin et al., 2021)

Gene

Coordinates: 2,851,283 → 2,852,140

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The protein

Catalyzed reaction/ biological activity

• H⁺ + prephenate --> 3-phenylpyruvate + CO₂ + H₂O (according to UniProt)

[wiki|Domains]

• Prephenate dehydratase domain (aa 2-183) (according to UniProt)
• [wiki|ACT domain] (aa 204-281) (according to UniProt)

Structure

• [PDB|4LUB] (from ''Streptococcus mutans'', 42% identity)
• [AF|P21203]

Effectors of protein activity

• subject to feedback inhibtion by phenylalanine [Pubmed|4956345]

Biological materials

Mutant

• 1A617 ( ''pheA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A617&Search=1A617 BGSC]
• BKE27900 (Δ[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GACTTTCATGACGATTTTCT,  downstream forward: _UP4_TAAAAAAAGCCCACTAGAGG
• BKK27900 (Δ[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27900 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GACTTTCATGACGATTTTCT,  downstream forward: _UP4_TAAAAAAAGCCCACTAGAGG

References

• 19258532,2537815,4956345