Pgi

• Description: glucose 6-phosphate isomerase, glycolytic / gluconeogenic enzyme
  
  

Gene name	pgi
Synonyms	yugL
Essential	no
Product	glucose-6-phosphate isomerase
Function	enzyme in glycolysis / gluconeogenesis
Gene expression levels in SubtiExpress: pgi
Metabolic function and regulation of this protein in SubtiPathways: pgi
MW, pI	50.4 kDa, 4.85
Gene length, protein length	1353 bp, 451 amino acids
Immediate neighbours	yugM, yugK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, phosphoproteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU31350

Database entries

• BsubCyc: BSU31350

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glucose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot) D-glucose 6-phosphate = D-fructose 6-phosphate

• Protein family: GPI family (according to Swiss-Prot) GPI family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • Reversible Michaelis-Menten PubMed
  • specific activity: 2.1 µmol min^-1 (mg protein)^-1 PubMed

• Domains:

• Modification: phosphorylation on Thr-39 PubMed

• Cofactors:

• Effectors of protein activity: competitively inhibited by 6-phosphogluconate (in B.caldotenax, B.stearothermophilus) PubMed

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

• BsubCyc: BSU31350

• Structure: 1B0Z (Geobacillus stearothermophilus), 2PGI (Geobacillus stearothermophilus)

• UniProt: P80860

• KEGG entry: [3]

• E.C. number: 5.3.1.9

Additional information

• extensive information on the structure and enzymatic properties of Pgi can be found at Proteopedia

Expression and regulation

• Operon: pgi-yugM-yugN PubMed

• Expression browser: pgi PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 5183 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 17347 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 7808 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 5723 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 7262 PubMed

Biological materials

• Mutant: GP508 (spc), available in Jörg Stülke's lab, PubMed

• Expression vector:
  • pGP398 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab

• lacZ fusion: pGP510 (in pAC6), available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany homepage