Difference between revisions of "PfkA"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 863 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 3832 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:50, 17 April 2014

  • Description: phosphofructokinase, glycolytic enzyme

Gene name pfkA
Synonyms pfk
Essential no
Product 6-phosphofructokinase
Function catabolic enzyme in glycolysis
Gene expression levels in SubtiExpress: pfkA
Interactions involving this protein in SubtInteract: PfkA
Metabolic function and regulation of this protein in SubtiPathways:
pfkA
MW, pI 34,1 kDa, 6.14
Gene length, protein length 957 bp, 319 amino acids
Immediate neighbours pyk, accA
Sequences Protein DNA DNA_with_flanks
Genetic context
PfkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PfkA expression.png















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU29190

Phenotypes of a mutant

  • essential according to PubMed, non-essential according to PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
  • Protein family: phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Allosteric Regulation (Reversible) PubMed
  • Domains:
    • 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
  • Modification:
    • phosphorylated on Arg-233 PubMed
  • Cofactor(s): Mg2+
  • Effectors of protein activity:
    • Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in B. licheniformes PubMed.
    • Inhibited by ATP (competitively) and f6p (non-competitively) in G. stearothermophillus PubMed
    • Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in G. stearothermophillus) PubMed
    • Activated by NH4+ PubMed

Database entries

  • Structure:
    • 4A3S PubMed
    • 1MTO (mutant, complex with fructose-6-phosphate, Geobacillus stearothermophilus)
    • 4PFK (Geobacillus stearothermophilus)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • twofold induced by glucose PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 863 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3832 PubMed

Biological materials

  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP87, available in Jörg Stülke's lab
    • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, in pGP382: pGP1266, available in Jörg Stülke's lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP393, available in Jörg Stülke's lab
    • for expression in B. subtilis, in pBQ200: pGP1422, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References