Difference between revisions of "PfeT"

From SubtiWiki
Jump to: navigation, search
m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk)
Line 100: Line 100:
 
* '''Operon:''' ''zosA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
 
* '''Operon:''' ''zosA'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zosA_1451371_1453284_1 zosA] {{PubMed|22383849}}
 +
 
 +
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
  
 
* '''Regulation:''' induced by hydrogen peroxide ([[PerR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]
 
* '''Regulation:''' induced by hydrogen peroxide ([[PerR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12180919 PubMed]

Revision as of 07:49, 13 April 2012

  • Description: zinc transporter

Gene name zosA
Synonyms ykvW
Essential no
Product P-type zinc-transporting ATPase
Function zinc uptake
MW, pI 68 kDa, 4.934
Gene length, protein length 1911 bp, 637 aa
Immediate neighbours stoA, ykvY
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkvW context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

  • Locus tag: BSU13850

Phenotypes of a mutant

    • reduced genetic competence, can be rescued by the addition of excess zinc PubMed
    • reduced expression of comK, control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase PubMed
  • Protein family: Type IB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation: induced by hydrogen peroxide (PerR) PubMed
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mitsuo Ogura
ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis.
J Biochem: 2011, 150(6);615-25
[PubMed:21813502] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mayuree Fuangthong, John D Helmann
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.
J Bacteriol: 2003, 185(21);6348-57
[PubMed:14563870] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis.
Mol Microbiol: 2002, 45(4);997-1005
[PubMed:12180919] [WorldCat.org] [DOI] (P p)