Difference between revisions of "Pel"

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|style="background:#ABCDEF;" align="center"|'''Function''' || degradation of polygalacturonic acid  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || degradation of polygalacturonic acid  
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07560 pel]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07560 pel]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 8.421   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 8.421   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflT]]'', ''[[yflS]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflT]]'', ''[[yflS]]''
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12585&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07560 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:pel_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pel_context.gif]]

Revision as of 12:21, 13 May 2013

  • Description: pectate lyase C

Gene name pel
Synonyms
Essential no
Product pectate lyase C
Function degradation of polygalacturonic acid
Gene expression levels in SubtiExpress: pel
MW, pI 45 kDa, 8.421
Gene length, protein length 1260 bp, 420 aa
Immediate neighbours yflT, yflS
Sequences Protein DNA Advanced_DNA
Genetic context
Pel context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Pel expression.png
























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, ComA regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU07560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to Swiss-Prot)
  • Protein family: polysaccharide lyase 1 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2O1D (bound to trisaccharide), 1BN8
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed by glucose (3.8-fold) (CcpA) PubMed
    • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • expressed at high cell density (ComA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications