pel

168

pectate lyase C

locus: BSU_07560

Molecular weight: 45.34 kDa

pI: 8.42

Protein length: 420 aa

Gene length: 1263 bp

function: degradation of polygalacturonic acid

product: pectate lyase C

essential: no

ec: 4.2.2.2

synonyms: pel

List of homologs in different organisms, belongs to COG3866 (Galperin et al., 2021)

Gene

Coordinates: 827,993  829,255

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The protein

Catalyzed reaction/ biological activity

• Eliminative cleavage of (14)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to UniProt)

Protein family

• [wiki|lyase 1 family] (according to UniProt)

Structure

• [PDB|2O1D] (bound to trisaccharide),  [PDB|1BN8], [PDB|5X2I]
• [AF|P39116]

[wiki|Localization]

• extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]

Biological materials

Mutant

• MGNA-C248 (pel::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2246 NBRP B. subtilis, Japan]
• BKE07560 ([gene|60595234B02D673E860B364FE8A3C83D2CC68CB2|pel]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCACTATGTACCCCCAT,  downstream forward: _UP4_TAAGAAAGTGAAAAACACAA
• BKK07560 ([gene|60595234B02D673E860B364FE8A3C83D2CC68CB2|pel]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCACTATGTACCCCCAT,  downstream forward: _UP4_TAAGAAAGTGAAAAACACAA

References

Reviews

• 20735481

Original Publications

• 8262178,16091051,12823818,18957862,12850135,25755103,7634076,26582911,27447541,27965289,36853818