pdxT

168

pyridoxal-5-phosphate synthase (glutaminase domain)

locus: BSU_00120

Molecular weight: 21.30 kDa

pI: 4.98

Protein length: 196 aa

Gene length: 591 bp

function: pyridoxal-5-phosphate biosynthesis

product: pyridoxal-5-phosphate synthase (glutaminase domain)

essential: no

ec: 4.3.3.6

synonyms: pdxT, yaaE

List of homologs in different organisms, belongs to COG0311 (Galperin et al., 2021)

Gene

Coordinates: 19,968  20,558

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth and colony morphology [Pubmed|28189581]
• auxotrophic for pyridoxal-5-phosphate at low external ammonium concentration [pubmed|33559288,14762015], this can be suppressed by overexpression of [gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS] or the ammonium transporter [protein|796BD46066B1E6147048664FA78679B42FA9D628|nrgA] [pubmed|33559288]
• inactivation of [gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT] reduces [wiki|sporulation] efficiency to 1.2% that of wild type cells; delayed entry into [wiki|sporulation] [Pubmed|26735940]
• poor growth [pubmed|28189581]
• non-transformable [pubmed|28189581]

Advertisement

The protein

Catalyzed reaction/ biological activity

• Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of [protein|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]. (according to UniProt)
• aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine --> H⁺ + 3 H₂O + L-glutamate + phosphate + pyridoxal 5'-phosphate (according to UniProt)

Protein family

• Glutaminase PdxT/SNO family (single member, according to UniProt)

Structure

• [PDB|2NV2] [protein|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[protein|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT] complex [Pubmed|17159152]
• [PDB|2NV0] (PdxT) [Pubmed|17159152]
• [AF|P37528]

[wiki|Localization]

• cytosol (according to UniProt)

Biological materials

Mutant

• BKE00120 ([gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT,  downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG
• BKK00120 ([gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT,  downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG
• BV604 (([gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT])::tet), available in [wiki|Fabian Commichau]'s lab,  [Pubmed|24972371]
• BP1100 (([gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT])::tet) trp⁺), available in [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs
• BP1105 (([gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT])::cat) trp⁺), available in [wiki|Jörg Stülke]'s and [wiki|Fabian Commichau]'s labs

References

Reviews

• 33665688

Original Publications

• 15911615,14762015,14651647,16030023,19152323,17159152,23832367,25473090,25568319,26490441,26735940,16233205,14585832,28189581,33559288