Pdp

• Description: pyrimidine nucleoside phosphorylase
  
  

Gene name	pdp
Synonyms
Essential	no
Product	pyrimidine nucleoside phosphorylase
Function	pyrimidine interconversion
Gene expression levels in SubtiExpress: pdp
Metabolic function and regulation of this protein in SubtiPathways: Nucleoside catabolism, Nucleotides (regulation)
MW, pI	46 kDa, 4.765
Gene length, protein length	1299 bp, 433 aa
Immediate neighbours	hutM, nupC
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

CcpA regulon, DeoR regulon

The gene

Basic information

• Locus tag: BSU39400

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A pyrimidine nucleoside + phosphate = a pyrimidine base + alpha-D-ribose 1-phosphate (according to Swiss-Prot)

• Protein family: thymidine/pyrimidine-nucleoside phosphorylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1BRW (Geobacillus stearothermophilus)

• UniProt: P39142

• KEGG entry: [3]

• E.C. number: 2.4.2.2

Expression and regulation

• Operon: dra-nupC-pdp PubMed

• Expression browser: pdp PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by deoxynucleotides (DeoR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • DeoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xianmin Zeng, Anne Galinier, Hans H Saxild
Catabolite repression of dra-nupC-pdp operon expression in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 11);2901-2908
[PubMed:11065368] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)

X Zeng, H H Saxild
Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis.
J Bacteriol: 1999, 181(6);1719-27
[PubMed:10074062] [WorldCat.org] [DOI] (P p)

H H Saxild, L N Andersen, K Hammer
Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein.
J Bacteriol: 1996, 178(2);424-34
[PubMed:8550462] [WorldCat.org] [DOI] (P p)