pdp

168

pyrimidine nucleoside phosphorylase

locus: BSU_39400

Molecular weight: 46.04 kDa

pI: 4.76

Protein length: 433 aa

Gene length: 1302 bp

function: pyrimidine interconversion

product: pyrimidine nucleoside phosphorylase

essential: no

ec: 2.4.2.2

synonyms: pdp

List of homologs in different organisms, belongs to COG0213 (Galperin et al., 2021)

Gene

Coordinates: 4,049,009 → 4,050,310

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The protein

Catalyzed reaction/ biological activity

• phosphate + uridine --> α-D-ribose 1-phosphate + uracil (according to UniProt)
• phosphate + thymidine --> 2-deoxy-α-D-ribose 1-phosphate + thymine (according to UniProt)
• 2'-deoxyuridine + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + uracil (according to UniProt)

Protein family

• thymidine/pyrimidine-nucleoside phosphorylase family (single member, according to UniProt)

Structure

• [PDB|5OLN] [PubMed|29633966]
• [AF|P39142]

Expression and Regulation

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• BKE39400 (Δ[gene|D2EB7BBAC8817912DAC4E215879F5A4E2C47ECAF|pdp]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGGTCACCGATCCT,  downstream forward: _UP4_TAAAAAATAAAGCACATCCC
• BKK39400 (Δ[gene|D2EB7BBAC8817912DAC4E215879F5A4E2C47ECAF|pdp]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTGGTCACCGATCCT,  downstream forward: _UP4_TAAAAAATAAAGCACATCCC

References

• 11065368,8550462,10074062,10666464,26883633,29633966,9817849