pdaA

168

N-acetylmuramic acid deacetylase, spore cortex peptidoglycan synthesis

locus: BSU_07980

Molecular weight: 29.92 kDa

pI: 7.32

Protein length: 263 aa

Gene length: 792 bp

function: spore cortex peptidoglycan synthesis

product: N-acetylmuramic acid deacetylase

essential: no

synonyms: pdaA, yfjS

List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)

Gene

Coordinates: 869,559  870,350

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The protein

Catalyzed reaction/ biological activity

• de-N-acetylation of N-acetylglucosamine and N-acetyl-muramic acid for spore cortex peptidoglycan [Pubmed|15687192]

Protein family

• [wiki|polysaccharide deacetylase family] (according to UniProt)

[wiki|Domains]

• [wiki|NodB homology domain] (aa 66-247) (according to UniProt)

Structure

• [PDB|1W17] [pubmed|15251431]
• [AF|O34928]

Biological materials

Mutant

• MGNA-C273 (yfjS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2271 NBRP B. subtilis, Japan]
• BKE07980 ([gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG,  downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC
• BKK07980 ([gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG,  downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC

References

• 12374835,15687192,15699190,15251431,14679227,24405365