Difference between revisions of "PdaA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || germination
 
|style="background:#ABCDEF;" align="center"|'''Function''' || germination
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07980 pdaA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07980 pdaA]
 
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|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 7.318   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 7.318   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfjT]]'', ''[[yfjR]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfjT]]'', ''[[yfjR]]''
 
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|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12627&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU07980 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU07980 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU07980 Advanced_DNA]
 
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|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yfjS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yfjS_context.gif]]

Revision as of 12:22, 13 May 2013

  • Description: polysaccharide deacetylase, required for germination

Gene name pdaA
Synonyms yfjS
Essential no
Product polysaccharide deacetylase
Function germination
Gene expression levels in SubtiExpress: pdaA
MW, pI 29 kDa, 7.318
Gene length, protein length 789 bp, 263 aa
Immediate neighbours yfjT, yfjR
Sequences Protein DNA Advanced_DNA
Genetic context
YfjS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PdaA expression.png
























Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU07980

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: polysaccharide deacetylase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Toshihiko Kitajima, Junichi Sekiguchi
A polysaccharide deacetylase homologue, PdaA, in Bacillus subtilis acts as an N-acetylmuramic acid deacetylase in vitro.
J Bacteriol: 2005, 187(4);1287-92
[PubMed:15687192] [WorldCat.org] [DOI] (P p)

David E Blair, Daan M F van Aalten
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine.
FEBS Lett: 2004, 570(1-3);13-9
[PubMed:15251431] [WorldCat.org] [DOI] (P p)

Meghan E Gilmore, Dabanjan Bandyopadhyay, Amanda M Dean, Sarah D Linnstaedt, David L Popham
Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan.
J Bacteriol: 2004, 186(1);80-9
[PubMed:14679227] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Hiroki Yamamoto, Abdelmadjid Atrih, Simon J Foster, Junichi Sekiguchi
A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis.
J Bacteriol: 2002, 184(21);6007-15
[PubMed:12374835] [WorldCat.org] [DOI] (P p)