Difference between revisions of "PdaA"

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(Expression and regulation)
(References)
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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:10, 1 August 2010

  • Description: polysaccharide deacetylase, required for germination

Gene name pdaA
Synonyms yfjS
Essential no
Product polysaccharide deacetylase
Function germination
MW, pI 29 kDa, 7.318
Gene length, protein length 789 bp, 263 aa
Immediate neighbours yfjT, yfjR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YfjS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU07980

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: polysaccharide deacetylase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Toshihiko Kitajima, Junichi Sekiguchi
A polysaccharide deacetylase homologue, PdaA, in Bacillus subtilis acts as an N-acetylmuramic acid deacetylase in vitro.
J Bacteriol: 2005, 187(4);1287-92
[PubMed:15687192] [WorldCat.org] [DOI] (P p)

David E Blair, Daan M F van Aalten
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine.
FEBS Lett: 2004, 570(1-3);13-9
[PubMed:15251431] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Hiroki Yamamoto, Abdelmadjid Atrih, Simon J Foster, Junichi Sekiguchi
A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis.
J Bacteriol: 2002, 184(21);6007-15
[PubMed:12374835] [WorldCat.org] [DOI] (P p)