Difference between revisions of "PcrA"
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' |
| + | ** PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop {{PubMed|20723756}} | ||
| + | ** dismantles [[RecA]] filaments {{PubMed|20723756}} | ||
* '''Protein family:''' uvrD-like helicase C-terminal domain (according to Swiss-Prot) | * '''Protein family:''' uvrD-like helicase C-terminal domain (according to Swiss-Prot) | ||
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<pubmed> 19943906 </pubmed> | <pubmed> 19943906 </pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>12073041,9701819 ,16267290, 19943900 </pubmed> | + | <pubmed>12073041,9701819 ,16267290, 19943900 20723756 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:49, 15 October 2010
- Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
| Gene name | pcrA |
| Synonyms | yerF |
| Essential | yes PubMed |
| Product | ATP-dependent DNA helicase |
| Function | plasmid rolling-circle replication, conjugative transfer of ICEBs1 |
| MW, pI | 83 kDa, 5.767 |
| Gene length, protein length | 2217 bp, 739 aa |
| Immediate neighbours | pcrB, ligA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU06610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure: 2PJR (�product complex�, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (�substrate complex�, complex with DNA, Geobacillus stearothermophilus)
- UniProt: O34580
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906]
[WorldCat.org]
[DOI]
(I p)
Original Publications
