PckA

• Description: phosphoenolpyruvate carboxykinase synthesises phosphoenolpyrovat out of oxalacetate
  
  

Gene name	pckA
Synonyms	ppc
Essential	no
Product	phosphoenolpyruvate carboxykinase
Function	synthesis of phosphoenolpyrovat
MW, pI	58,1 kDa, 5.12
Gene length, protein length	1581 bp, 527 amino acids
Immediate neighbours	metK, ytmB
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)

• Protein family: phosphoenolpyruvate carboxykinase [ATP] family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • Nucleotide binding Domain (233–240)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon:

• Sigma factor: SigA PubMed

• Regulation: neg. regulated by YqzB PubMed, repressed (35-times) Glc, repressor CcpN

• Regulatory mechanism:

• Additional information:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

1. Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. PubMed
2. Tännler et al. (2008) CcpN controls central carbon fluxes in Bacillus subtilis. J. Bacteriol. 190: 6178-6187. PubMed