Difference between revisions of "PckA"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
(Biological materials)
Line 113: Line 113:
 
** pGP1753 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
 
** pGP1753 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
 
** pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Stülke]] lab)  
 
** pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Stülke]] lab)  
 +
** pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag in [[pGP172]], available in [[Stülke]] lab)
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 14:09, 4 November 2010

  • Description: phosphoenolpyruvate carboxykinase

Gene name pckA
Synonyms ppc
Essential no
Product phosphoenolpyruvate carboxykinase
Function synthesis of phosphoenolpyruvate
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 58,1 kDa, 5.12
Gene length, protein length 1581 bp, 527 amino acids
Immediate neighbours metK, ytmB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PckA context.gif
This image was kindly provided by SubtiList








The gene

Basic information

  • Locus tag: BSU30560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
  • Protein family: phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Nucleotide binding Domain (233–240)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
    • Mdh-PckA (this interaction occurs under gluconeogenic, but not under glycolytic conditions) PubMed
  • Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

  • Structure: 2PXZ (E.coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP1753 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
    • pGP1762 (for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Stülke lab)
    • pGP1763 (for expression, purification in E. coli with N-terminal Strep-tag in pGP172, available in Stülke lab)
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:
  • FLAG-tag construct: GP1102 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References