Difference between revisions of "PckA"

From SubtiWiki
Jump to: navigation, search
Line 83: Line 83:
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2PXZ 2PXZ] (''E.coli'')
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2PXZ 2PXZ] (''E.coli'')
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P54418 P54418]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P54418 P54418]
  
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30560]
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30560]

Revision as of 14:13, 20 July 2009

  • Description: phosphoenolpyruvate carboxykinase

Gene name pckA
Synonyms ppc
Essential no
Product phosphoenolpyruvate carboxykinase
Function synthesis of phosphoenolpyruvate
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 58,1 kDa, 5.12
Gene length, protein length 1581 bp, 527 amino acids
Immediate neighbours metK, ytmB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PckA context.gif
This image was kindly provided by SubtiList








The gene

Basic information

  • Locus tag: BSU30560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
  • Protein family: phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Nucleotide binding Domain (233–240)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

  • Structure: 2PXZ (E.coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: repressed by glucose (7-fold) PubMed, repressed (35-times) Glc, repressor CcpN PubMed
  • Regulatory mechanism: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J Bacteriol: 2008, 190(18);6178-87
[PubMed:18586936] [WorldCat.org] [DOI] (I p)

Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol Microbiol: 2005, 55(5);1435-51
[PubMed:15720552] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)