PbuX

• Description: xanthine transport in/out via proton symport
  
  

Gene name	pbuX
Synonyms	ypaQ
Essential	no
Product	xanthine permease
Function	xanthine uptake
Gene expression levels in SubtiExpress: pbuX
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation)
MW, pI	46 kDa, 8.793
Gene length, protein length	1314 bp, 438 aa
Immediate neighbours	bcsA, xpt
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

• Locus tag: BSU22060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• Structure:

• UniProt: P42086

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: xpt-pbuX PubMed

• Expression browser: pbuX PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of adenine or adenosine (PurR) PubMed
  • induced in the absence of guanine (G-box) PubMed

• Regulatory mechanism:
  • G-box: transcription termination/ antitermination (riboswitch) PubMed
  • PurR: transcription repression (molecular inducer: PRPP) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J Bacteriol: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

L C Christiansen, S Schou, P Nygaard, H H Saxild
Xanthine metabolism in Bacillus subtilis: characterization of the xpt-pbuX operon and evidence for purine- and nitrogen-controlled expression of genes involved in xanthine salvage and catabolism.
J Bacteriol: 1997, 179(8);2540-50
[PubMed:9098051] [WorldCat.org] [DOI] (P p)