Difference between revisions of "PbuO"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytjP]]'', ''[[ythQ]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytjP]]'', ''[[ythQ]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29990 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29990 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29990 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29990 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ytiP_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ytiP_context.gif]]

Revision as of 11:02, 14 May 2013

Gene name pbuO
Synonyms ytiP
Essential no
Product unknown
Function unknown
Gene expression levels in SubtiExpress: pbuO
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 45 kDa, 7.484
Gene length, protein length 1296 bp, 432 aa
Immediate neighbours ytjP, ythQ
Sequences Protein DNA DNA_with_flanks
Genetic context
YtiP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PbuO expression.png




























Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

PurR regulon

The gene

Basic information

  • Locus tag: BSU29990

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): PbuG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Regulatory mechanism:
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J Bacteriol: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)

D J Ebbole, H Zalkin
Interaction of a putative repressor protein with an extended control region of the Bacillus subtilis pur operon.
J Biol Chem: 1989, 264(6);3553-61
[PubMed:2536750] [WorldCat.org] (P p)