Difference between revisions of "PbuG"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''pbuG''
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
* '''Regulation:''' repressed by hypoxanthine and guanine, [[PurR]]-independent [http://www.ncbi.nlm.nih.gov/sites/entrez/12923093 PubMed]
+
* '''Regulation:'''  
 +
** repressed by hypoxanthine and guanine, [[PurR]]-independent, ([[G-box]])[http://www.ncbi.nlm.nih.gov/sites/entrez/12923093 PubMed]
  
* '''Regulatory mechanism:''' riboswitch [http://www.ncbi.nlm.nih.gov/sites/entrez/12923093 PubMed]
+
* '''Regulatory mechanism:''' [[G-box]]: transcription termination/ antitermination ([[riboswitch]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12923093 PubMed]
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 20:30, 31 May 2009

  • Description: hypoxanthin/ guanine permease

Gene name pbuG
Synonyms yebB
Essential no
Product hypoxanthin/ guanine permease
Function hypoxanthine and guanine uptake
MW, pI 46 kDa, 8.808
Gene length, protein length 1320 bp, 440 aa
Immediate neighbours guaA, yebC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PbuG context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): PbuO

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: membrane PubMed

Database entries

  • Structure: 2EEW (the pbuG riboswitch, U47c mutant, bound to hypoxanthine)
  • Swiss prot entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon: pbuG
  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
  2. Johansen et al. (2003) Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL). J. Bacteriol. 185: 5200-5209. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed