PbpB

• Description: class B penicillin-binding protein 2B, Pbp2B
  
  

Gene name	pbpB
Synonyms
Essential	yes PubMed
Product	penicillin-binding protein 2B
Function	septation, cell division
Gene expression levels in SubtiExpress: pbpB
Interactions involving this protein in SubtInteract: PbpB
MW, pI	79 kDa, 9.234
Gene length, protein length	2148 bp, 716 aa
Immediate neighbours	ftsL, spoVD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, cell division, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15160

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15160

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: transpeptidase family (according to Swiss-Prot)

• Paralogous protein(s): SpoVD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • folding requires PrsA PubMed
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-PbpB PubMed

• Localization:
  • cell membrane PubMed
  • division sites PubMed
  • extracellular (signal peptide) PubMed
  • localizes to the sporulation septum during sporulation PubMed

Database entries

• BsubCyc: BSU15160

• Structure: 1RP5 (PBP 2x from Streptococcus pneumoniae, 32% identity) PubMed

• UniProt: Q07868

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mraZ-mraW-ftsL-pbpB PubMed

• Expression browser: pbpB PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• FLAG-tag construct:
  • GP2003 pbpB-3xFLAG spec (based on pGP1331), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage

References

Katharina Peters, Inga Schweizer, Katrin Beilharz, Christoph Stahlmann, Jan-Willem Veening, Regine Hakenbeck, Dalia Denapaite
Streptococcus pneumoniae PBP2x mid-cell localization requires the C-terminal PASTA domains and is essential for cell shape maintenance.
Mol Microbiol: 2014, 92(4);733-55
[PubMed:24655324] [WorldCat.org] [DOI] (I p)

Marta Carolina Afonso Lages, Katrin Beilharz, Danae Morales Angeles, Jan-Willem Veening, Dirk-Jan Scheffers
The localization of key Bacillus subtilis penicillin binding proteins during cell growth is determined by substrate availability.
Environ Microbiol: 2013, 15(12);3272-81
[PubMed:23895585] [WorldCat.org] [DOI] (I p)

Tatsuya Fukushima, Isako Furihata, Robyn Emmins, Richard A Daniel, James A Hoch, Hendrik Szurmant
A role for the essential YycG sensor histidine kinase in sensing cell division.
Mol Microbiol: 2011, 79(2);503-22
[PubMed:21219466] [WorldCat.org] [DOI] (I p)

Susan L Rowland, Kimberly D Wadsworth, Scott A Robson, Carine Robichon, Jon Beckwith, Glenn F King
Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B.
J Bacteriol: 2010, 192(23);6116-25
[PubMed:20870765] [WorldCat.org] [DOI] (I p)

Hanne-Leena Hyyryläinen, Bogumila C Marciniak, Kathleen Dahncke, Milla Pietiäinen, Pascal Courtin, Marika Vitikainen, Raili Seppala, Andreas Otto, Dörte Becher, Marie-Pierre Chapot-Chartier, Oscar P Kuipers, Vesa P Kontinen
Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis.
Mol Microbiol: 2010, 77(1);108-27
[PubMed:20487272] [WorldCat.org] [DOI] (I p)

Pamela Gamba, Jan-Willem Veening, Nigel J Saunders, Leendert W Hamoen, Richard A Daniel
Two-step assembly dynamics of the Bacillus subtilis divisome.
J Bacteriol: 2009, 191(13);4186-94
[PubMed:19429628] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Lucile Pernot, Laurent Chesnel, Audrey Le Gouellec, Jacques Croizé, Thierry Vernet, Otto Dideberg, Andréa Dessen
A PBP2x from a clinical isolate of Streptococcus pneumoniae exhibits an alternative mechanism for reduction of susceptibility to beta-lactam antibiotics.
J Biol Chem: 2004, 279(16);16463-70
[PubMed:14734544] [WorldCat.org] [DOI] (P p)

Dirk-Jan Scheffers, Laura J F Jones, Jeffery Errington
Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis.
Mol Microbiol: 2004, 51(3);749-64
[PubMed:14731276] [WorldCat.org] [DOI] (P p)

R A Daniel, E J Harry, J Errington
Role of penicillin-binding protein PBP 2B in assembly and functioning of the division machinery of Bacillus subtilis.
Mol Microbiol: 2000, 35(2);299-311
[PubMed:10652091] [WorldCat.org] [DOI] (P p)

R A Daniel, A M Williams, J Errington
A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.
J Bacteriol: 1996, 178(8);2343-50
[PubMed:8636036] [WorldCat.org] [DOI] (P p)

A Yanouri, R A Daniel, J Errington, C E Buchanan
Cloning and sequencing of the cell division gene pbpB, which encodes penicillin-binding protein 2B in Bacillus subtilis.
J Bacteriol: 1993, 175(23);7604-16
[PubMed:8244929] [WorldCat.org] [DOI] (P p)