pabA

168

para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II)

locus: BSU_00750

Molecular weight: 21.54 kDa

pI: 4.78

Protein length: 194 aa

Gene length: 585 bp

function: biosynthesis of folate and tryptophan

product: para-aminobenzoate synthase (subunit B)/ anthranilate synthase (subunit II) glutamine amidotransferase (subunit B) and anthranilate synthase (subunit II)

essential: no

ec: 2.6.1.85

synonyms: pabA, trpG, trpX

List of homologs in different organisms, belongs to COG0512 (Galperin et al., 2021)

Gene

Coordinates: 84,290  84,874

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The protein

Catalyzed reaction/ biological activity

• Chorismate + L-glutamine --> anthranilate + pyruvate + L-glutamate (according to UniProt)

[wiki|Domains]

• [wiki|Glutamine amidotransferase type-1 domain] (aa 1-194) (according to UniProt)

Structure

• [PDB|1I1Q] (from ''Salmonella typhimurium'', 42% identity) [Pubmed|11224570]
• [AF|P28819]

Expression and Regulation

Other regulations

• [protein|E030E80134E9DED6ADBC73C7C7435024CDFD38B9|mtrB]: translation inhibition, [Pubmed|9084182]

additional information

• the [gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]-[gene|865678B10097C40337F5EEA77FF0F6E7BF5358DC|pabC] part of the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• BKE00750 ([gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATCATTTCTCCGC,  downstream forward: _UP4_TATCGCAAGGAAGTTATTGC
• BKK00750 ([gene|956E33AAAADB302EB8663E4B75C917242D3AD2C0|pabA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAAAATCATTTCTCCGC,  downstream forward: _UP4_TATCGCAAGGAAGTTATTGC

References

Reviews

• 16285852,19385727

Original Publications

• 8647825,7515880,9084182,17114263,21815947,11224570