PWH844

pWH844

vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein

the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Hillen lab

in E. coli: ampicillin resistance

similar plasmid; pGP570

Schirmer et al. (1997) J. Bacteriol. 179: 1329-1336. PubMed