PHT43

From SubtiWiki
Revision as of 11:34, 28 August 2017 by Bzhu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Disclaimer

The content of this page/section is provided by a third party. SubtiWiki acknowledges that this third party content is obtained from a source believed to be reliable. Please note that no guarantees are made by SubtiWiki as to reliability of this third party information. Provision of this third party content is for general information purpose and does not constitute a recommendation. SubtiWiki does not own copyright over this third party content (including but not limited to text, images, and media).

PHT43.PNG PHT43-Legende.jpg

The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

Features

  • Secretory protein production
  • Efficiently controllable gene expression (IPTG inducible)
  • High level production and secretion of recombinant proteins
  • E. coli / B. subtilis shuttle vector

pHT43 and the eight-fold protease-deficient B. subtilis strain WB800N, for secretory protein production are available from MoBiTec GmbH (Göttingen)Mobiteclogo.png

References

Hoang Duc Nguyen, Trang Thi Phuong Phan, Wolfgang Schumann
Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis.
Curr Microbiol: 2007, 55(2);89-93
[PubMed:17624574] [WorldCat.org] [DOI] (P p)

Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann
Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis.
Protein Expr Purif: 2006, 46(2);189-95
[PubMed:16125412] [WorldCat.org] [DOI] (P p)

Hoang Duc Nguyen, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann
Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.
Plasmid: 2005, 54(3);241-8
[PubMed:16005967] [WorldCat.org] [DOI] (P p)