Difference between revisions of "PHT43"

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The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.
 
The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.

Revision as of 09:23, 28 August 2017

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PHT43.PNG PHT43-Legende.jpg

The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

Features

  • Secretory protein production
  • Efficiently controllable gene expression (IPTG inducible)
  • High level production and secretion of recombinant proteins
  • E. coli / B. subtilis shuttle vector

pHT43 and the eight-fold protease-deficient B. subtilis strain WB800N, for secretory protein production are available from MoBiTec GmbH (Göttingen)

References