http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT254&feed=atom&action=history
PHT254 - Revision history
2024-03-28T20:44:11Z
Revision history for this page on the wiki
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http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT254&diff=209764&oldid=prev
Bzhu at 11:35, 28 August 2017
2017-08-28T11:35:55Z
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<td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 11:35, 28 August 2017</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l14" >Line 14:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* ''E. coli / B. subtilis'' shuttle vector</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* ''E. coli / B. subtilis'' shuttle vector</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The pHT254 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The pHT254 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)<ins class="diffchange diffchange-inline">][[File:Mobiteclogo.png|100px]</ins>]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div><pubmed>16125412, 22100269, 25990516</pubmed></div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div><pubmed>16125412, 22100269, 25990516</pubmed></div></td></tr>
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Bzhu
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT254&diff=209751&oldid=prev
Bzhu at 09:25, 28 August 2017
2017-08-28T09:25:06Z
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<td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 09:25, 28 August 2017</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l2" >Line 2:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[File:PHT254.PNG|600px]]</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[File:PHT254.PNG|600px]]</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:<del class="diffchange diffchange-inline">Legend</del>.<del class="diffchange diffchange-inline">png</del>|<del class="diffchange diffchange-inline">230px</del>]]</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:<ins class="diffchange diffchange-inline">PHT254-Legende</ins>.<ins class="diffchange diffchange-inline">jpg</ins>|<ins class="diffchange diffchange-inline">300px</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>The pHT254 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant C-terminal His tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.  </div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>The pHT254 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant C-terminal His tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.  </div></td></tr>
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Bzhu
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT254&diff=209720&oldid=prev
Bzhu: Created page with "{{ContentDisclamer}} 600px 230px The pHT254 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular ..."
2017-08-21T13:45:08Z
<p>Created page with "{{ContentDisclamer}} <a href="/wiki//index.php/File:PHT254.PNG" title="File:PHT254.PNG">600px</a> <a href="/wiki//index.php/File:Legend.png" title="File:Legend.png">230px</a> The pHT254 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular ..."</p>
<p><b>New page</b></p><div>{{ContentDisclamer}}<br />
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[[File:PHT254.PNG|600px]]<br />
[[File:Legend.png|230px]]<br />
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The pHT254 vector contains the coding sequence for an 8xHis tag and allows high-level intracellular production of recombinant C-terminal His tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest. <br />
The expression is controlled by the strong P''grac100'' promoter, consisting of the ''groeESL'' promoter of ''B. subtilis'' with improved regulatory elements fused to the ''lac'' operator allowing their induction by IPTG. Nucleotides were optimized at the conserved regions of the ''groESL'' promoter including the UP element, the −35 and the −15 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein of ''B. subtilis'' (Phan ''et al''., 2011). pHT254 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.<br />
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=== Features ===<br />
* Strong promoter with improved regulatory elements<br />
* Enhanced amount of produced recombinant proteins<br />
* IPTG inducible gene expression <br />
* C-terminal 8xHis tag<br />
* ''E. coli / B. subtilis'' shuttle vector<br />
<br />
The pHT254 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]<br />
<br />
== References ==<br />
<br />
<pubmed>16125412, 22100269, 25990516</pubmed></div>
Bzhu