http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT10&feed=atom&action=history
PHT10 - Revision history
2024-03-28T20:03:43Z
Revision history for this page on the wiki
MediaWiki 1.30.0
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT10&diff=209762&oldid=prev
Bzhu at 11:35, 28 August 2017
2017-08-28T11:35:31Z
<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr style="vertical-align: top;" lang="en">
<td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 11:35, 28 August 2017</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l14" >Line 14:</td>
<td colspan="2" class="diff-lineno">Line 14:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* ''E. coli / B. subtilis'' shuttle vector</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>* ''E. coli / B. subtilis'' shuttle vector</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>pHT10 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>pHT10 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)<ins class="diffchange diffchange-inline">][[File:Mobiteclogo.png|100px]</ins>]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>== References ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div><pubmed>17624574, 16005967, 16125412</pubmed></div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div><pubmed>17624574, 16005967, 16125412</pubmed></div></td></tr>
</table>
Bzhu
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT10&diff=209748&oldid=prev
Bzhu at 09:22, 28 August 2017
2017-08-28T09:22:56Z
<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
<col class="diff-marker" />
<col class="diff-content" />
<col class="diff-marker" />
<col class="diff-content" />
<tr style="vertical-align: top;" lang="en">
<td colspan="2" style="background-color: white; color:black; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: white; color:black; text-align: center;">Revision as of 09:22, 28 August 2017</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l2" >Line 2:</td>
<td colspan="2" class="diff-lineno">Line 2:</td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[File:PHT10.PNG|600px]]</div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>[[File:PHT10.PNG|600px]]</div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>[[File:<del class="diffchange diffchange-inline">Legend</del>.<del class="diffchange diffchange-inline">png</del>|<del class="diffchange diffchange-inline">230px</del>]]</div></td><td class='diff-marker'>+</td><td style="color:black; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>[[File:<ins class="diffchange diffchange-inline">PHT10-Legende</ins>.<ins class="diffchange diffchange-inline">jpg</ins>|<ins class="diffchange diffchange-inline">300px</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.  </div></td><td class='diff-marker'> </td><td style="background-color: #f9f9f9; color: #333333; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #e6e6e6; vertical-align: top; white-space: pre-wrap;"><div>The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.  </div></td></tr>
</table>
Bzhu
http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PHT10&diff=209714&oldid=prev
Bzhu: Created page with "{{ContentDisclamer}} 600px 230px The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular pro..."
2017-08-21T13:35:49Z
<p>Created page with "{{ContentDisclamer}} <a href="/wiki//index.php/File:PHT10.PNG" title="File:PHT10.PNG">600px</a> <a href="/wiki//index.php/File:Legend.png" title="File:Legend.png">230px</a> The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular pro..."</p>
<p><b>New page</b></p><div>{{ContentDisclamer}}<br />
<br />
[[File:PHT10.PNG|600px]]<br />
[[File:Legend.png|230px]]<br />
<br />
The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest. <br />
The expression is based on the strong σA-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT10 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis.''<br />
<br />
=== Features ===<br />
<br />
* High level production of recombinant proteins <br />
* Efficiently controllable gene expression (IPTG inducible)<br />
* C-terminal c-Myc tag<br />
* ''E. coli / B. subtilis'' shuttle vector<br />
<br />
pHT10 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]<br />
<br />
== References ==<br />
<pubmed>17624574, 16005967, 16125412</pubmed></div>
Bzhu