Difference between revisions of "PHT01"

From SubtiWiki
Jump to: navigation, search
Line 4: Line 4:
 
[[File:Legend.png|230px]]
 
[[File:Legend.png|230px]]
  
The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
+
The pHT01 is a high-level intracellular expression vector for recombinant protein production with <i>B. subtilis</i>. The vector is based on the strong σ<sup>A</sup>-dependent promoter preceding the ''[[groES]]-[[groEL]]'' operon of <i>B. subtilis</i> which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an <i>E. coli/ B. subtilis</i> shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
  
 
=== Features ===
 
=== Features ===

Revision as of 13:00, 21 August 2017

Disclaimer

The content of this page/section is provided by a third party. SubtiWiki acknowledges that this third party content is obtained from a source believed to be reliable. Please note that no guarantees are made by SubtiWiki as to reliability of this third party information. Provision of this third party content is for general information purpose and does not constitute a recommendation. SubtiWiki does not own copyright over this third party content (including but not limited to text, images, and media).

PHT01.PNG Legend.png

The pHT01 is a high-level intracellular expression vector for recombinant protein production with B. subtilis. The vector is based on the strong σA-dependent promoter preceding the groES-groEL operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.

Features

  • Efficiently controllable gene expression (IPTG inducible)
  • High level production of recombinant proteins
  • E. coli / B. subtilis shuttle vector

pHT01 and further pHT-vector variants (e.g., with His, Strep or Myc tag) are available from MoBiTec GmbH (Göttingen)

References

Hoang Duc Nguyen, Trang Thi Phuong Phan, Wolfgang Schumann
Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis.
Curr Microbiol: 2007, 55(2);89-93
[PubMed:17624574] [WorldCat.org] [DOI] (P p)

Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann
Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis.
Protein Expr Purif: 2006, 46(2);189-95
[PubMed:16125412] [WorldCat.org] [DOI] (P p)

Hoang Duc Nguyen, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann
Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.
Plasmid: 2005, 54(3);241-8
[PubMed:16005967] [WorldCat.org] [DOI] (P p)