PGP380

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pGP380: click to enlarge
pGP380 cloning region: click to enlarge

Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP382

sequencing primer:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘


Christina Herzberg, Lope Andrés Flórez Weidinger, Bastian Dörrbecker, Sebastian Hübner, Jörg Stülke, Fabian M Commichau
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo.
Proteomics: 2007, 7(22);4032-5
[PubMed:17994626] [WorldCat.org] [DOI] (P p)