PGP380

From SubtiWiki
Revision as of 13:41, 8 May 2009 by Jstuelk (talk | contribs)
Jump to: navigation, search
pGP380: click to enlarge
pGP380 cloning region: click to enlarge

Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP382

sequencing primer:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘


Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed