Difference between revisions of "PGP380"

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Line 8: Line 8:
 
in ''E. coli'': ampicillin resistance
 
in ''E. coli'': ampicillin resistance
  
in B''. subtilis'': erythromycin resistance
+
in ''B. subtilis'': erythromycin resistance
  
 
based on [[pBQ200]]
 
based on [[pBQ200]]

Revision as of 11:23, 16 April 2009

pGP380: click to enlarge
pGP380 cloning region: click to enlarge

Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP382

sequencing primer:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘


Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed