Difference between revisions of "PGP380"
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[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']] | [[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']] | ||
| − | Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology | + | Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the [[SPINE]] technology |
constructed in [[Stülke]] lab | constructed in [[Stülke]] lab | ||
Revision as of 13:41, 8 May 2009
Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology
constructed in Stülke lab
in E. coli: ampicillin resistance
in B. subtilis: erythromycin resistance
based on pBQ200
similar vector: pGP382
sequencing primer:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed
