PGP172

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pGP172: click to enlarge
pGP172 cloning region: click to enlarge

pGP172

vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP574

sequencing primer:

  • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
  • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’


Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) Mycoplasma pneumoniae HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. PubMed