Difference between revisions of "PGP172"

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(Undo revision 121172 by Fabian Commichau (Talk))
(Undo revision 121171 by Fabian Commichau (Talk))
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[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
 
[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
  
'''Applications:'''
 
  
Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C. pGP172 is similar [[plasmid]] to [[pGP574]].
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vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
  
Sequencing primers:  
+
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 +
 
 +
constructed in the [[Stülke]] lab
 +
 
 +
in E. coli: ampicillin resistance
 +
 
 +
based on pET3C
 +
 
 +
similar [[plasmid]]: [[pGP574]]
 +
 
 +
sequencing primer:  
 
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  
 
<pubmed> 14717704 </pubmed>
 
<pubmed> 14717704 </pubmed>

Revision as of 10:18, 14 April 2010

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP574

sequencing primer:

  • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
  • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)