Difference between revisions of "PGP172"

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vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
+
vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
  
 
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
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based on pET3C
 
based on pET3C
  
similar plasmid: [[pGP574]]
+
similar [[plasmid]]: [[pGP574]]
  
 
sequencing primer:  
 
sequencing primer:  

Revision as of 11:59, 16 April 2009

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP574

sequencing primer:

  • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
  • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’


Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) Mycoplasma pneumoniae HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. PubMed