Difference between revisions of "PGP172"
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed) |
|||
| Line 1: | Line 1: | ||
| − | vector for | + | pGP172 |
| − | constructed in the | + | |
| + | vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein | ||
| + | |||
| + | host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
| + | |||
| + | constructed in the [[Stülke]] lab | ||
| + | |||
in E. coli: ampicillin resistance | in E. coli: ampicillin resistance | ||
| − | + | ||
| − | based on -- | + | based on pET3C |
| − | + | ||
| + | Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) ''Mycoplasma pneumoniae'' HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. [http://www.ncbi.nlm.nih.gov/sites/entrez/14717704 PubMed] | ||
Revision as of 11:21, 15 January 2009
pGP172
vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
Merzbacher, M., Detsch, C., Hillen, W. & Stülke, J. (2004) Mycoplasma pneumoniae HPr kinase/ phosphorylase: Assigning functional roles to the P-loop and HPr kinase/ phosphorylase signature sequence motif. Eur. J. Biochem. 271: 367-374. PubMed
