Difference between revisions of "PGP172"

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[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
 
[[File:PGP172_cloning_region.jpeg|250px|thumb|right|'''pGP172 cloning region: click to enlarge''']]
  
 +
'''Applications:'''
  
vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
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Vector pGP172 was designed for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein. The ''E. coli'' strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in ''E. coli''. The plasmid was constructed in the [[Stülke]] lab and it is based on the vector pET3C. pGP172 is similar [[plasmid]] to [[pGP574]].
  
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
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Sequencing primers:  
 
 
constructed in the [[Stülke]] lab
 
 
 
in E. coli: ampicillin resistance
 
 
 
based on pET3C
 
 
 
similar [[plasmid]]: [[pGP574]]
 
 
 
sequencing primer:  
 
 
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  
 
<pubmed> 14717704 </pubmed>
 
<pubmed> 14717704 </pubmed>

Revision as of 14:57, 25 February 2010

pGP172: click to enlarge
pGP172 cloning region: click to enlarge

Applications:

Vector pGP172 was designed for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein. The E. coli strain BL21(DE3)/pLysS serves as a host strain and the expression of the desired protein is induced by the addition of 1 mM IPTG to the culture. pGP172 confers resistance to ampicillin in E. coli. The plasmid was constructed in the Stülke lab and it is based on the vector pET3C. pGP172 is similar plasmid to pGP574.

Sequencing primers:

  • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
  • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)