Difference between revisions of "PGP172"
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*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | *'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’ | ||
| − | + | <pubmed> 14717704 </pubmed> | |
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Revision as of 18:37, 7 September 2009
vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
in E. coli: ampicillin resistance
based on pET3C
sequencing primer:
- CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
- NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704]
[WorldCat.org]
[DOI]
(P p)
