Difference between revisions of "PGP1460"
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[[File:PGP1460.png|250px|thumb|right|'''pGP1460: click to enlarge''']] | [[File:PGP1460.png|250px|thumb|right|'''pGP1460: click to enlarge''']] | ||
| + | [[File:PGP382_cloning_region.jpeg|250px|thumb|right|'''pGP382 cloning region: click to enlarge''']] | ||
The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method. | The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like [[pGP382]] it can be used for the [[SPINE]] method. | ||
Revision as of 12:26, 16 May 2012
The vector was constructed in the Stülke lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the SPINE method.
Detailed information about the multiple cloning site can be found here: pGP382.
Genes cloned into this vector require a Shine-Dalgarno sequence.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
Similar plasmid: pGP1459 (for N-terminal Strep-tags)
