Difference between revisions of "PGP1459"
| Line 8: | Line 8: | ||
Sequencing primers: | Sequencing primers: | ||
| − | *''' | + | *'''KG64:''' 5‘-TATCAGGGCCTCGACTACA-3‘ |
| − | *''' | + | *'''ML107:''' 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘ |
Similar plasmid: [[pGP1460]] (for C-terminal Strep-tags) | Similar plasmid: [[pGP1460]] (for C-terminal Strep-tags) | ||
<pubmed> </pubmed> | <pubmed> </pubmed> | ||
Revision as of 15:25, 2 May 2013
The vector was constructed in the Stülke lab and it is suitable for constitutive expression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. The plasmid will integrate into the ganA gene. Just like pGP380 it can be used for the SPINE method.
The cloning region of pGP380 is shown for detailed information.
Sequencing primers:
- KG64: 5‘-TATCAGGGCCTCGACTACA-3‘
- ML107: 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘
Similar plasmid: pGP1460 (for C-terminal Strep-tags)
