PBP137

From SubtiWiki

Revision as of 17:15, 29 October 2013 by Kgunka (talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to: navigation, search

pBP137: click to enlarge

pBP137 cloning region: click to enlarge

Vector for the construction of translational gfp fusions that can be integrated at the amyE site

Does not replicate in B. subtilis, integrates into the amyE site

Constructed in Fabian Commichau's lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenicol resistance

similar vectors: pAC6, pAC7

sequencing primers:

KG307: 5’-CACCTTCACCCTCTCCACTGACAG -3’
PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘

Retrieved from "http://subtiwiki.uni-goettingen.de/wiki//index.php?title=PBP137&oldid=194429"