Difference between revisions of "PBP137"
(Created page with "Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site Does not replicate in ''B. subtilis'', integrates into the ''amyE'' s...") |
|||
| Line 1: | Line 1: | ||
| + | [[File:Map_pBP137.png|250px|thumb|right|'''pBP137: click to enlarge''']] | ||
| + | |||
| + | |||
Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site | Vector for the construction of translational ''gfp'' fusions that can be integrated at the ''amyE'' site | ||
Revision as of 16:12, 29 October 2013
Vector for the construction of translational gfp fusions that can be integrated at the amyE site
Does not replicate in B. subtilis, integrates into the amyE site
Constructed in Fabian Commichau's lab
- in E. coli: ampicillin resistance
- in B. subtilis: chloramphenicol resistance
sequencing primers:
- KG307: 5’-CACCTTCACCCTCTCCACTGACAG -3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
