Difference between revisions of "PAC6"

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constructed in the Rapoport lab
 
constructed in the Rapoport lab
  
in E. coli: ampicillin resistance
+
in ''E. coli'': ampicillin resistance
  
in B. subtilis: chloramphenocol resistance
+
in ''B. subtilis'': chloramphenocol resistance
  
 
similar vectors: [[pAC5]], [[pAC7]]
 
similar vectors: [[pAC5]], [[pAC7]]

Revision as of 11:22, 16 April 2009

pAC6: click to enlarge

pAC6

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenocol resistance

similar vectors: pAC5, pAC7

sequencing primer:

  • PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
  • PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
  • Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’


Stülke J, Martin-Verstraete I, Zagorec M (1997) Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT Mol Microbiol. 25: 65-78. PubMed