Difference between revisions of "PAC6"
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[[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] | [[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] | ||
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vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
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in ''E. coli'': ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
| − | in ''B. subtilis'': | + | in ''B. subtilis'': chloramphenicol resistance |
similar vectors: [[pAC5]], [[pAC7]] | similar vectors: [[pAC5]], [[pAC7]] | ||
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*'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | ||
| − | + | <pubmed> 11902727 </pubmed> | |
| − | + | == Return to [[Plasmids]] == | |
Latest revision as of 14:53, 3 June 2013
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenicol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
J Stülke, I Martin-Verstraete, M Zagorec, M Rose, A Klier, G Rapoport
Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT.
Mol Microbiol: 1997, 25(1);65-78
[PubMed:11902727]
[WorldCat.org]
[DOI]
(P p)
