Difference between revisions of "PAC6"

From SubtiWiki
Jump to: navigation, search
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed)
 
Line 1: Line 1:
vector for
+
pAC6
constructed in the --- lab
+
 
 +
vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site
 +
 
 +
does not replicate in ''B. subtilis'', integrates into the ''amyE'' site
 +
 
 +
constructed in the Rapoport lab
 +
 
 
in E. coli: ampicillin resistance
 
in E. coli: ampicillin resistance
in B. subtilis: ---- resistance
+
 
based on ---
+
in B. subtilis: chloramphenocol resistance
reference. PubMed
+
 
 +
similar vectors: [[pAC5]], [[pAC7]]
 +
 
 +
Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. [http://www.ncbi.nlm.nih.gov/sites/entrez/1619665  PubMed]

Revision as of 10:04, 15 January 2009

pAC6

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenocol resistance

similar vectors: pAC5, pAC7

Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. PubMed