PAC5

pAC5: click to enlarge

pAC5 cloning region: click to enlarge

pAC5

vector for the construction of translational lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenicol resistance

similar vectors: pAC6, pAC7

sequencing primer:

• PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
• PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
• Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G: (1992) Mutagenesis of the Bacillus subtilis ‘-12, -24’ promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol , 226:85-99. PubMed